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81.
Thakur D Rejtar T Wang D Bones J Cha S Clodfelder-Miller B Richardson E Binns S Dahiya S Sgroi D Karger BL 《Journal of chromatography. A》2011,1218(45):8168-8174
Precise proteomic profiling of limited levels of disease tissue represents an extremely challenging task. Here, we present an effective and reproducible microproteomic workflow for sample sizes of only 10,000 cells that integrates selective sample procurement via laser capture microdissection (LCM), sample clean-up and protein level fractionation using short-range SDS-PAGE, followed by ultrasensitive LC-MS/MS analysis using a 10 μm i.d. porous layer open tubular (PLOT) column. With 10,000 LCM captured mouse hepatocytes for method development and performance assessment, only 10% of the in-gel digest, equivalent to ~1000 cells, was needed per LC-MS/MS analysis. The optimized workflow was applied to the differential proteomic analysis of 10,000 LCM collected primary and metastatic breast cancer cells from the same patient. More than 1100 proteins were identified from each injection with >1700 proteins identified from three LCM samples of 10,000 cells from the same patient (1123 with at least two unique peptides). Label free quantitation (spectral counting) was performed to identify differential protein expression between the primary and metastatic cell populations. Informatics analysis of the resulting data indicated that vesicular transport and extracellular remodeling processes were significantly altered between the two cell types. The ability to extract meaningful biological information from limited, but highly informative cell populations demonstrates the significant benefits of the described microproteomic workflow. 相似文献
82.
83.
The effects of RGD peptide conjugation to alginate hydrogel on the adipogenic differentiation of ASCs was investigated. After 3 d of culture, RGD-modified alginate hydrogels significantly stimulated FAK and integrin α1 gene expressions and vinculin expression in ASCs. In addition, RGD-modified alginate hydrogels significantly enhanced the adipogenic differentiation of human ASCs to exhibit higher expression levels of oil red O staining and adipogenic genes compared to those of the control group (unmodified gels). These results suggest potential applications of RGD-modified alginate gels for adipose tissue regeneration. 相似文献
84.
In many cases, the survival probability of a system depends not only on the intrinsic characteristic of the system itself
but also on the randomly variable external environment under which the system is being operated. In this paper we study a
stochastic survival model for a system under random shock process which affects the survival of the system in a complicated
way. The lifetime distribution of the system is derived, and the effect of environmental factors on the failure process of
the system is also investigated. 相似文献
85.
86.
87.
R. Kalfat N. Gharbi S. Romdhane J.-L. Fave A. Chaïeb M. Majdoub 《Journal of Sol-Gel Science and Technology》1998,13(1-3):439-443
Photoluminescence of a polysiloxane gel film incorporating a partially conjugated copolymer polyether-polyphenylenevinylene (PEPPV) has been studied and compared with results obtained from bulk and vacuum evaporated films of the same starting material. The evaporation clearly affects the distribution of lengths of the conjugated parts, yet the incorporation in the gel matrix induces only slight changes in the spectroscopic properties of the polymer. 相似文献
88.
高功率激光稳定腔选模分析 总被引:1,自引:0,他引:1
讨论了在有限菲涅耳数时,稳定凹-凸腔的高功率激光选模特性,研究表明当稳定凹-凸腔工作在临近界稳区时,基模体积显著提高,具有良好的选模效果。 相似文献
89.
An interferometric concept based on a modified version of the Fabry–Perot interferometry is proposed for measuring flow fields in real-time and some results are presented for demonstration. Being based on a single-path configuration of interfering beams, it is very convenient to build a simple compact measurement system. While allowing the deployment of low-quality optics including test-section windows through compensation of system errors, it is also immune to external vibration. In addition, it can easily allow large aperture and long pathlength of probing beams with good precision. These aforementioned features are very appropriate for measuring large flow fields including wind tunnel testing as well as conducting laboratory experiments without elaborate equipment. 相似文献
90.
A new pteridine compound was isolated from green sulfur photosynthetic bacteria, Chlorobium limicola f. thiosulfatophilum NCIB 8327. The structure of this pterin derivative was established to be 1-O-(L -erythro-5,6,7,8- tetrahydropterin-2′-yl)-β-N-acetylglucosamine ( 1 ) from 1H-NMR and CD spectra as well as from various mass spectrometric techniques and chemical-cleavage techniques. Upon acid hydrolysis of 1 , equimolar amounts of biopterin ( 2 ) and N-acetylglucosamine were produced. The structure of the hydrolysis product 2 was confirmed by comparing its NMR, UV, CD, and MS and its chromatographical behavior with those of an authentic specimen. N-Acetylglucosamine was identified by an enzymatic hydrolysis experiment as well as by NMR and thin layer chromatography. Electrospray (ES), fast-atom-bombardment (FAB), and thermospray (TS) mass spectrometry of 1 yielded an MH+ at m/z 441. Periodate-oxidation experiments of the intact molecule 1 and of its hydrolysis product 2 are consistent with the proposed structure. Differential I2 oxidation experiments with the native compound showed that the in vivo oxidation state of this pterin is its tetrahydro form. We propose the trivial name ‘limipterin’ for this new compound. 相似文献